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Restructuring Institute Rudjer Boskovic

A group of Rudjer Boskovic Institutes' (RBI) scientists has proposed a programme under the title RBI-BAR for restructuring the Molecular Medicine Department within RBI. Ruđer Bošković Institute for Biomedical and Applied Research (RBI-BAR) has set the goal to steer biomedical research into new avenues of innovation and technology, and to become the leading institute for basic and applied research in Biomedicine in Croatia and South Eastern Europe. The mission is "to provide innovative molecular solutions for major diseases of the world through scholarship and collaboration". RBI-BAR will promote the innovative use of genetic and genomic approaches and technologies for basic science discoveries and clinical research applications for the prevention, diagnosis, and treatment of relevant human diseases with focus on cancer.

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Scienion Spotter robot sciFLEXARRAYER S3

The sciFLEXARRAYER S3 is an automated piezo driven non-contact dispensing system of ultra-low volumes.The spotter is a versatile instrument that enables fabrication of different types of custom arrays, mostly protein or DNA microarrays of custom design for specific needs of the scientific community. Potential applications are listed below.

Applications

  • DNA, protein, glycan arraying and biosensor loading
  • Cell transfection arrays
  • MALDI-MS sample preparation and target loading
  • Accurate dilution series and addition of tiny aliquots
  • Printing chemical libraries
  • Spotting onto disc format (round targets) and customized targets
  • Assay development and screening assays
  • Microarray-based analysis

Technical features

sciFlexarrayer
Dispense volume
 50-800 pl per drop
Capillary orifice 50-100 μm
Capillary material
 Borosilicate glass
Typical spot size
 80-250 μm
Typical pitch (spacing)
 300 μm (scaleable)

 

 

Agilent SureScan Microarray Scanner

The microarray scanner is a sophisticated laser- induced fluorescence scanner designed to read microarrays printed on standard 1 x 3" (25 mm x 75 mm) glass slides. The microarray scanner measures the fluorescence intensity of labeled sample nucleic acid (DNA and RNA), and protein bound to microarrays. Compatible dyes and excitation wavelengths are listed in table below. Its ability to measure fluorescence from two dyes simultaneously facilitates all two- color microarray studies. This technology provides for rapid, high- quality, automated scanning of microarrays.

Technical features

Microarray Scanner                                      
Compatible Dyes

Cyanine 3 and Cyanine 5, Alexa 647, 555, and 660
Laser Information

HG-YAG laser (532nm), Helium-Neon laser (633nm)
Glass Dimensions Supported 1 x 3" (25 mm x 75 mm) glass slide
Width: 24.95 mm to 26.1 mm
Length: 74.8 mm to 76.45 mm
Thickness: 0.9 mm to 1.1mm
Non-Mirrored slides of low intrinsic fluorescence

 

Agilent 2100 Bioanalyzer

The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells on a single platform. Results are delivered within 30-40 minutes in automated, high quality digital data.

Technical features

Bioanalyzer

Protein                                       

SDS-PAGE replacement for protein analysis

Determine the quantity and purity of proteins in the range of 5 - 230 kD
Applications
- Analyze protein expression
- Analyze protein purification
- Protein validation and QC
- Analyze protein degradation

 
RNA

Applications
- Analyze and quantify low concentrated RNA samples down to 50 pg/µl.
- Resolve small nucleic acids in the size range of 6 to 150 nt.
- Check total RNA integrity and quantify total RNA and mRNA.

DNA

Applications

- Automated sizing and quantification of PCR fragments, restriction digests or fragmented DNA.

 

 
This project is funded by the  
European Union  
  A project implemented by the
 Ruđer Bošković Institute