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Restructuring Institute Rudjer Boskovic

A group of Rudjer Boskovic Institutes' (RBI) scientists has proposed a programme under the title RBI-BAR for restructuring the Molecular Medicine Department within RBI. Ruđer Bošković Institute for Biomedical and Applied Research (RBI-BAR) has set the goal to steer biomedical research into new avenues of innovation and technology, and to become the leading institute for basic and applied research in Biomedicine in Croatia and South Eastern Europe. The mission is "to provide innovative molecular solutions for major diseases of the world through scholarship and collaboration". RBI-BAR will promote the innovative use of genetic and genomic approaches and technologies for basic science discoveries and clinical research applications for the prevention, diagnosis, and treatment of relevant human diseases with focus on cancer.

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Human ORFeome Collection

Human ORFeome Collection (hORFeome) represents an ultimate library of cloned human genes (>17000).

Custom microarray fabrication, and biosensor design will rely on the hORFeome collection recently established at IRB. It will serve as an extremely efficient shortcut as almost every known human gene will be available for microarray fabrication and, subsequent R&D activities and/or prototype development. Enabling such technology will be of high impact for the scientific community in Croatia and will increase R&D potential and innovative capacity. Due to the extreme simplification of the cloning processes by using the Gateway cloning system, the Translational Medicine Group (TMG) can offer subcloning of the genes of interest into appropriate expression vectors prior to array spotting. The combination of state-of-the-art equipment, personell expertise and almost unlimited access to thousands of human genes makes TMG one of the most perspective core-facilities in the region.

  • Easy-to-transfer – Gateway©-adapted
  • Maximum flexibility – Stop codon removed to allow fusion-tags

Browse the hORFeome collection to find desired clones


hORFeome clones are derived from fully sequenced

Mammalian Gene Collection (MGC) full-length cDNAs and cloned

into a recombinational entry vector.


Time Savings

ORF clones provide a shortcut to protein expression, allowing you

to skip PCR, cloning into an expression vector, and verifying the

ends of the ORF DNA sequence.


Ease Of Transfer

The Gateway entry vectors ensure easy transfer into prokaryotic,

mammalian, viral, or insect expression systems. For maximum

flexibility, the ORF stop codon has been removed.


References and links:

1. The completion of the Mammalian Gene Collection. Genome Research 2009;19:2324-33

This article cites 35 articles, 21 of which can be accessed free at:


2. hORFeome 3.1: a resource of human open reading frames covering over 10,000 human genes. Genomics 2007;89:307-15

3. Towards a proteome-scale map of the human protein-protein interaction network. Nature 437:1173-8, 2005

Human ORFeome Collection (Openbiosystems)


This project is funded by the  
European Union  
 A project implemented by the
 Ruđer Bošković Institute