Microarray Data and Analysis (scheduled for late 2011)
IRB TransMed platform using high resolution microarray scanning technology will feature:
DNA and protein array scanning
- Provide users DNA and Protein array Core Facility operations for pay-for-use-basis.
The list of available DNA arrays is growing rapidly, facilitating prokaryotic and eukaryotic research, from bacteria, fungi, plants, insects, fish to mammals and others. So is the list of applications for DNA microarrays, and examples are diagnostics of diseases, screening programmes, gene regulation and expression, miRNA research etc.
Microarray applications are in brief:
• DNA Methylation
Discover and monitor epigenetic modifications known to play a fundamental role in many cellular processes such as cancer, neurodegenerative disease etc.
o Study methylation changes across the genome.
• Chromatin Immunoprecipitation (ChIP-on-chip)
o Elucidate the role that protein-DNA interactions play in processes such as transcription, replication, modification and repair.
• Gene Expression analysis
Explore gene transcription on a genome-wide basis across a variety of model systems. Conducting:
o basic research
o identifying new drug targets
o discovering biomarkers
• Comparative Genomic Hybridization (oligo aCGH)
Conduct high-resolution, genome-wide profiling of DNA copy number changes associated with cancer and other genetic diseases.
o Pinpoint chromosomal aberrations with array technology instead of time consuming FISH analysis.
o Identify boundaries of chromosome aberrations
o Detect low copy number changes
o Detect small regions of deletion
o Precisely define chromosome breakpoints
• Splice Variant Analysis
Perform global interrogations of the transcriptome and identify alternative splice forms to uncover the role gene variants play in drug response and disease.
• MicroRNA identification
Profile microRNA’s (miRNA’s) and explore the role they play in gene regulation.
GMP
Good manufacturing procedures (GMP) will be applied to producing the highest quality product. All fabrication is completed in climate-controlled laboratory where our scientists use good laboratory practices (GLP) for sample handling, laboratory control, personal safety protection, and process control to achieve the best results for your project. IRB TransMed supports our clients through each stage of regulatory approval processes including site certification requirements.
Action staff will assist users by:
• Provide assistance to customers in experiment design
• Order commercial arrays
• Produce custom arrays
• Perform RNA quality check and RIN value determination.
• Perform array analysis
• Perform initial bioinformatics evaluation
• Issue analysis report
• Issue bill for services
